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KMID : 0880220080460020209
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Journal of Microbiology
2008 Volume.46 No. 2 p.209 ~ p.213
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Phage Types and Pulsed-Field Gel Electrophoresis Patterns of Salmonella enterica serovar Enteritidis Isolated from Humans and Chickens
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Kim Sung-Hun
Kim Shuk-Ho
Park Mi-Sun
Lee Bok-Kwon
Park Jeong-Hyun
Chun Sung-Guen
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Abstract
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We analyzed 66 Salmonella Enteritidis isolates in 2002. Thirty isolates were obtained from human patients with diarrhea, and 36 were obtained from chickens. A total of ten phage types (PT) were identified in the human and chicken isolates. PT1 and PT21 were the predominant PTs in both the human (20% and 13%) and chicken (17% and 47%) isolates. Twelve pulsotypes were generated by PFGE and divided into two major groups. Most of the PFGE types were categorized into cluster group 1. Eighteen chicken isolates in cluster group 1 showed high-level genetic association (>95%) with 22 other human isolates. Additionally, six chicken isolates from cluster group 2 showed fairly high-level genetic association (>95%) with the other seven human isolates. The highest levels of genetic association in humans and chickens were seen with A5-PT21 (11 isolates), A2-PT1 (7 isolates), and B1-PT4 (6 isolates). The Pulsed-Field Gel Electrophoresis (PFGE) and phage typing provided conclusive evidence that human Salmonella infections are attributable to the consumption of contaminated chicken.
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KEYWORD
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Salmonella Enteritidis, pulsed-field gel electrophoresis, phage typing, resistance
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